1. Cultivation of Bt cotton has been much in news. The prefix "Bt" means

2. Bt toxin genes have been expressed in plants in order to provide resistance against

(i) fungi                                                                    (ii) animals and bacteria

(iii) bacteria and fungi                                           (iv) coleopterans and dipterans

(v) lepidopterans

3. Study the following statements regarding Bt toxins produced by bacterium Bacillus thuringiensis and select the correct one(s).

4. Bt toxin protein crystals present in bacterium Bacillus thuringiensis, do not kill the bacteria themselves because

5. Bt toxin kills insects by

6. Select the correct match(es).

Gene                                                                                     Target insect

A. crylAc                                                                          Cotton bollworm

B. cryllAb                                                                         Com borer

C. crylAb                                                                           Cotton bollworm

7. Bt corn has been made resistant from corn borer disease by introduction of the gene

8. The process of RNA interference has been used to make tobacco plant resistant to

9. RNA interference involves

10. Nematode-specific genes are introduced into the tobacco plant by using Agrobacterium vectors to make it resistant. The introduced DNA in the host cells produces

11. How many recombinant therapeutics worldwide have been approved for human use?

12. Which of the following statements is not correct?

13. Which of the following statements regarding the structure of proinsulin and mature insulin are not correct?

(i) Proinsulin is made up of three polypeptide chains-A, B and C.

(ii) C-polypeptide chain is removed prior to insulin formation.

(iii) Mature insulin is arranged in two polypeptide chains -  A and B.

(iv) Polypeptide chains A and B are interconnected by only one S - S linkage.

14. Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of

14. Arrange the steps involved in the production of human insulin in the correct sequence and select the correct option.

(i) Synthesis of gene (DNA) for human insulin artificially

(ii) Culturing recombinant E.coli in bioreactors

(iii) Purification of human insulin

(iv) Insertion of human insulin gene into plasmid

(v) Introduction of recombinant plasmid into E.coli

(vi) Extraction of recombinant gene product from E.coli

16. Which of the following statements is incorrect about gene therapy for ADA deficiency?

17. An example of gene therapy is

18. Which of the following statements is not correct?

19. Select the correct option for the given statements.

(i) A transgenic cow, Rosie, produced human protein- enriched milk, which was nutritionally more balanced product for human babies than natural cow milk.

(ii) Milk produced by transgenic cow, Rosie, contained 2.4 gm protein/litre.

(iii) Milk produced by transgenic cow contained human alpha lactalbumin.

20. Which of the following statements is/are incorrect?

(i) Around 200,000 varieties of rice is estimated in India alone.

(ii) Less than 20 documented varieties of Basmati rice are grown in India.

(iii) Some nations are developing laws to prevent unauthorised exploitation of their bio-resources and traditional knowledge.

Assertion & Reason Questions

Directions: In the following questions, a statement of assertion is followed by a statement of reason. Mark the correct choice as:

21. Assertion: Human insulin can be produced using bacterial cells.

Reason: To produce human insulin, the A, B and C polypeptides of the human insulin are produced separately in the bacterial cells, extracted and combined by creating disulphide bonds.

Assertion & Reason Questions

Directions: In the following questions, a statement of assertion is followed by a statement of reason. Mark the correct choice as:

22. Assertion: ELISA is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins, glycoproteins, etc.) on it.

Reason: PCR is a powerful technique to identify many genetic disorders.

Statement Based Questions

Directions: In the following questions, read the given statements and select the correct option as:

23. Statement I: Transgenic mice are being developed for use in testing the safety of polio vaccine.

Statement II: Transgenic animals are made that carry genes which make them more sensitive to toxic substances than non-transgenic animals.

24. Match column I with column II and select the correct option from the given codes.

Column I                                                Column II

A. Biopiracy                                         (i)Effort to fix the non- functional gene

B. Biopatent                                         (ii) Gene silencing

C. Gene therapy                                  (iii) Illegal use of biological materials

D. RNAi                                                (iv) Right granted for biological entities

25. Match column I with column II and choose the correct option.

Column I                                                                                       Column II

A. α-1-antitrypsin                                                                  (i) Emphysema

B. Rosie                                                                                    (ii) Polio vaccine

C. Transgenic mice                                                                 (iii) Alpha-lactalbumin

D. Human protein                                                                   (iv) First transgenic cow