1. The term 'recombinant DNA' refers to

2. The first recombinant DNA was constructed when

3. Plasmid used to construct the first recombinant DNA was isolated from which bacterium species?

4. In 1972, S. Cohen and H. Boyer accomplished

5. The term 'molecular scissors' refer to

6. The different steps of genetically modifying an organism are given below.

A. Introduction of the identified DNA into the host.

B. Identification of DNA with desirable genes.

C. Transfer of the DNA to its progeny.

D. Maintenance of introduced DNA in the host.

Which of the following represents the correct sequence of steps?

7. Select the option which is not considered a key tool in recombinant DNA technology?

1. Host organism

2. DNA Ligase

3. Polymerase enzymes

4. Restriction enzymes

5. Vectors

8. Hindll cuts DNA molecule at a specific point by recognising a specific sequence of

9. The letter 'R' in EcoRI is derived from

10. Which of the following correctly depicts the recognition site for EcoRI?

11. Identify the DNA segment which is not a palindromic sequence.

12. Sticky ends of vector and foreign DNA are joined by

13.Gel electrophoresis is a

14. Read the following statements and select the correct ones.

(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge.

(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double stranded DNA molecules found naturally in many bacteria and also in some yeasts.

(iii) Exonuclease enzyme is generally not used while producing a recombinant DNA molecule.

(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13.

15. Name the fluorescent  intercalating dye used to stain DNA.

16. During elution ____(i)_____ coloured bands of DNA, are observed in a gel stained with _____(ii)_____ dye, under _______(iii)___ and _____(iv)________ to be extracted with an elution kit.

17. Directions: In the following questions, a statement of assertion is followed by a statement of reason. Mark the correct choice as:

Assertion : A piece of DNA inserted into an alien organism generally does not replicate if not inserted into a chromosome.

Reason: Chromosomes have specific sequences called 'ori' region where DNA replication is initiated.

18. Assertion: Hindll recognise a specific sequence of 5 base pairs.

Reason: Hindll is the first restriction endonuclease.

19. Assertion: DNA fragments move towards the anode under an electric field.

Reason: Smaller the DNA fragment size the farther it moves.

Q. no. 20 to 21.  Statement Based Questions Directions: In the following questions, read the given statements and select the correct option as:

20. Statement I: Restriction endonuclease enzymes recognise a specific palindromic nucleotide sequence in the DNA.

Statement II: Restriction endonuclease enzymes are called molecular scissors or biological scissors.

Q. no. 20 to 21.  Statement Based Questions Directions: In the following questions, read the given statements and select the correct option as:

21. Statement I: The overhanging stretches produced on DNA strands after action of restriction enzymes are called sticky-ends.

Statement II: Agarose gel used in gel electrophoresis is extracted from fungal species.

22. Matching Type Questions.

Match column I with column II with respect to the nomenclature of restriction enzyme EcoRI and select the correct answer from the given codes.

Column I                                                                             Column II

A. E                                                                              (i) I^st in order of isolation

B. CO                                                                           (ii) Name of genus

C. R                                                                              (iii) Name of species

D. I                                                                               (iv) Name of strain

23. An enzyme catalysing the removal of nucleotides from the ends of DNA is

24. Which of the given statements is correct in the context of observing DNA separated by agarose gel electrophoresis?

25. Which of the following is not required in the preparation of a recombinant DNA molecule ?